ABSTRACT
@#AIM:To investigate the neuroprotective effect of vitreous cavity transplantation of bone marrow mesenchymal stem cells on rat glaucoma model. <p>METHODS: Rat primary bone marrow mesenchymal stem cells were extracted and identified; Lewis rats were randomly divided into 3 groups: Control group, Glaucoma group and bone marrow mesenchymal stem cell group(BMMSCs group), and rat glaucoma model were made: the left eye was the experimental eye(glaucoma), the right eye is the control eye, and identified them. Then vitreous cavity transplantation of bone marrow mesenchymal stem cells were performed; HE staining was used to observe the morphology of the retina; The number of retinal ganglion cells was detected by immunofluorescence; TUNEL staining was used to observe the apoptosis of retinal ganglion cells; The expression of IGF1 and BDNF protein in retinal tissue was detected by Western blot. <p>RESULTS: The intraocular pressure of the experimental eyes of the rats was significantly higher than that of the control eyes(<i>P</i><0.01), indicating that the glaucoma rat models were successfully constructed; In the Glaucoma group, the retinal nerve fibers were irregularly arranged, the number of retinal ganglion cells and retinal thickness were significantly lower than that of the control group(<i>P</i><0.01), and the number of apoptosis of retinal ganglion cells was significantly higher than that of the control group(<i>P</i><0.001); The retinal nerve fiber cells in the BMMSCs group were arranged neatly. The number of retinal ganglion cells and retinal thickness were significantly higher than those in the Glaucoma group(<i>P</i><0.05), and the number apoptosis of retinal ganglion cells was significantly lower than that of Glaucoma group(<i>P</i><0.01); The expression of IGF1 and BDNF in the retina of the Glaucoma group was significantly lower than that in the control group(<i>P</i><0.01), the expression of IGF1 and BDNF in the retina of the BMMSCs group was higher than that in the Glaucoma group(<i>P</i><0.05). <p>CONCLUSION:Bone marrow mesenchymal stem cell vitreous cavity transplantation can improve glaucoma in rats and protect retinal ganglion cells.
ABSTRACT
Objective To investigate the expressions of miR-20a-5p/miR-20b-5p in gastric cancer cells and gastric carcinoma tissues,analyze their target genes and enriching signal pathways using bioinformatics methods,and explore their biological behavior and function.Methods The expression levels of miR-20a-5p/miR-20b-5p in gastric cancer cells with different differentiation such as high,middle or low differentiation,normal gastric mucosa cells,gastric cancer tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR,and their clinical significance was analyzed.The target genes of miR-20a-5p/miR-20b-5p were predicted using 10 softwares affiliated to mirWALK web database,and the genes supported by more than three softwares were selected as target genes.The signal pathways of target genes were enriched by online DAVID 6.7 software.Results The expression levels of miR-20a-5p/miR-20b-5p in gastric cancer cells with different differentiation were significantly higher than that in normal gastric mucosa cells (all P ＜0.05),and that in gastric cancer tissues higher than adjacent tissues (P ＜ 0.05).The up-regulated expression of miR-20b-5p was closely related to lymph node metastasis and invasion depth (all P ＜ 0.05).Bioinformatics analysis showed that the enriched target genes of miR-20a-5p/miR-20b-5p existed in multiple signaling pathways associated with cancer.Conclusion MiR-20a-5p/miR-20b-5p may be a promising biomarker of gastric cancer,which is highly expressed in gastric cancer and is related to lymph node metastasis and invasion depth.